Sensitivity & Specificity of ELISA Kit for Antibody Detection of Canine Parvovirus and Canine Distemper Infection in Dogs

¹Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Egypt

²Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Lahore, Pakistan

*Corresponding author: Hassenin ASH, Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Egypt, Tel: +1 6127706945; E-mails: [email protected]; [email protected].

Received Date: November 06, 2023

Published Date: December 22, 2023

Citation: Hassenin ASH, et al. (2023). Sensitivity & Specificity of ELISA Kit for Antibody Detection of Canine Parvovirus and Canine Distemper Infection in Dogs. Mathews J Vet Sci. 7(6):34.

Copyrights: Hassenin ASH, et al. © (2023).

ABSTRACT

Canine parvovirus (CPV) and canine distemper virus (CDV) are the common fetal disease of canine. Their control and prevention of these diseases include vaccination and high care should be done is important in canine shelters throughout the world. ELISA is a sero-chemical reaction for the helps for the detection of protective. Antibodies of these against both above viruses. The study helps to identify the goal was to confirm the specificity and sensitivity of ELISA and in comparison, compare it with with CPV hemagglutination inhibition and CDV serum neutralization test. s using sera collected from dogs housed at animal shelters. The ELISA was used under both field and laboratory conditions and duplicate specimens were processed using an extra wash step. The accuracy of test kits in serum samples results showed that the sensitivity of CDV was 94.0%, using an optical density meter 88.1% with a specificity of 91.8%. For CPV, the sensitivity was 92.3% with 93.5% specificity. This test appears to be a good indicator for antibody detection against both infections.

Keywords: ELISA, Titers, Virus, Canine, Sensitivity, Specificity.