The Rapid and Sensitive Hplc-Ms/Ms-Method of Determination of Mebeverine Metabolites in Human Plasma

¹State Budget-funded Educational Establishment of Higher Professional Education, Yaroslavl State Medical University of the Ministry of Health of the Russian Federation, Yaroslavl, Russia

²Quinta-Analytica Yaroslavl LLC, Yaroslavl, Russia.

³State Educational Establishment of Higher Professional Education, Yaroslavl State University named after P. G. Demidov of the Ministry of Education and Science of the Russian Federation, Yaroslavl, Russia.

Corresponding Author: Ilya Igorevich Yaichkov, 150043, Avtozavodskaya st, 29-97, Yaroslavl, Russian, Tel: 89109777498; E-Mail:  [email protected]

Received Date: 10 Feb 2017  
Accepted Date: 14 Feb 2017  
Published Date: 27 Feb 2017

Copyright © 2017 Yaichkov II

Citation: Khokhlov AL, Dzhurko YA, Yaichkov II, Shitov LN, et al. (2017). The Rapid and Sensitive Hplc-Ms/Ms-Method of Determination of Mebeverine Metabolites in Human Plasma.Mathews J Pharm Sci. 2(1): 010.

ABSTRACT:

The rapid and sensitive method for quantifying mebeverine metabolites in human plasma was developed using HPLC-MS/ MS. Mebeverine acid-D5 and desmethyl mebeverine acid-D5 were used as internal standards. Sample preparation was performed by protein precipitation. The chromatographic separation was achieved on Phenomenex Luna C8 Mercury (20 x 4.0 mm, 5 μm) and Phenomenex Luna 5u C8 (150 x 4.6 mm, 5 μm) under gradient conditions of mobile phase. The calibration range of mebeverine acid and desmethyl mebeverine acid was 10 - 2000 ng/ml. The method was applied to conduct a pharmacokinetic study of 200 mg prolonged-release capsules Duspatalin on 24 healthy participants.

KEYWORDS:

HPLC-MS/MS; Mebeverine Acid; Desmethyl Mebeverine Acid; Pharmacokinetic.