Duodenal-Jejunal Bypass Surgery Increased GLP-1 Receptor Expression in Enteric Nervous System of STZ-Induced Diabetic Rats

¹Department of Pharmacology, Key lab of Applied Pharmacology in University of Shandong, Weifang Medical University, Weifang, 261053, China.

²Department of Pediatric Surgery, Weifang People's Hospital, China.

³Affiliated Hospital of Weifang Medical University, China.

Corresponding Author: Mei-Hua Qu, Key Lab of Applied Pharmacology in University of Shandong, Weifang Medical University, Weifang, 261053, China, Tel:086-536-8462466; E-Mail: [email protected]

Received Date: 19 Sep 2016 
Accepted Date: 29 Dec 2016
Published Date: 03 Jan 2017

Copyright © 2016 Gao Z

Citation: Qu MH, Gao Z, Yan Q, Pan X, et al. (2016). Duodenal-jejunal bypass surgery increased GLP-1 receptor expression in enteric nervous system of STZ-induced diabetic rats. Mathews J  Diabetes Obes 2(1): 004.

ABSTRACT

Aims: Studies have shown that the Duodenal-jejunal bypass (DJB) is highly effective treatment of type 2 diabetes (T2DM) which glucagon like pipetide-1 (GLP-1) plays an important role. This study, we aimed to study DJB surgery effect on the expression of GLP-1R in the enteric nerve system and outset of type 2 diabetic rats.
Methods: Type 2 diabetic rats were generated by feeding Wistar rats with high-fat diet and low dose streptozotocin (STZ) injection. DJB and sham operations were performed in T2DM rats. Wistar rats were used as the wild-type control. Fasting blood glucose, insulin and GLP-1 secretion were measured before and eight weeks after surgery.Double-labeling immunofluorescence assay was performed to determine the changesof β cells (insulin) and α cells (glucagon) in pancreatic islets eight weeks after operation. Immunofluorescence and western blotting were performed to determine the expression of GLP-1R in Myenteric plexus (MP) of ENS after operation.
Results: DJB decreased the level of fasting blood glucose and the insulin secretion compared to the sham operation. Furthermore, DJB increased the β cell quantities in the pancreas compared to the sham group rats. DJB increased plasma GLP-1 secretionin T2DM rats comparing the sham rats. Immunofluorescence and Western blot results showed that GLP-1R expression in ENS was significantly increased eight weeks after DJB.
Conclusion: DJB improved the expression of GLP-1R in the enteric nerve system.And DJB increased GLP-1 secretion and improved the glucose disposal in T2DM and increased the quantity of β cells in the pancreas of T2DM rats.

KEYWORDS

Type 2 diabetes; Duodenal-jejunal bypass; Glucagon-like peptide-1; Glucagon-like peptide-1 receptor; enteric nervous system.