Evaluation of p53, Ki-67 and c-erb-B2 Expression in Normal Oral Epithelium, Dysplastic Epithelium, and Oral Squamous Cell Carcinoma

¹Associate Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ege.

²Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ege University, Bornova, Izmir, Turkey.

³Associate Professor, Department of Pathology, Faculty of Medicine, Ege University, Bornova, Izmir, Turkey.

⁴Professor, Department of Pathology, Faculty of Medicine, Ege University, Bornova, Izmir,Turkey.

⁵Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ege University, Bornova, Izmir, Turkey.

Corresponding Author: Banu Ozveri Koyuncu, Associate Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ege, Tel: 90-232-3881108; E-Mail: [email protected]

Received Date: 02 Oct 2018  
Accepted Date: 09 Oct 2018   
Published Date: 11 Oct 2018
Copyright © 2018 Koyuncu BO

Citation: Koyuncu BO, Akay C, Yaman B, Veral A, et al. (2018). Evaluation of p53, Ki-67 and c-erb-B2 expression in normal oral epithelium, dysplastic epithelium, and oral squamous cell carcinoma. Mathews J Dentistry. 3(1): 019.

ABSTRACT

The expression of p53, Ki-67, and c-erb-B2 in normal oral epithelium, dysplastic epithelium, and oral squamous cell carcinoma (OSCC) was investigated with immunohistochemical method and was determined the correlation between them. The study was performed on tissue sections of dysplasia (n= 25), OSCC (n=22). Twenty normal samples of oral epithelium were used as control. Sections were stained with immunohistochemically with antibodies to p53, Ki-67, and c-erb-B2 proteins. The percentage of positive cells was assessed in oral dysplastic, OSSC and normal epithelium. Statistical analysis was done using Kruskal-Wallis, Mann Whitney U, and the chi-square tests. The percentage of p53 and Ki-67 positive cells was evaluated and statistically significant differences were found between normal oral mucosa and dysplasia, and between dysplasia and invasive oral squamous cell carcinoma (p<0.05). C-erb-B2 positive cells increased from normal oral epithelium through dysplasia to OSCC, but it was not statistically important (p>0.05). A significant correlation between p53 and Ki-67 positive cells was observed (p<0.001) but no significant correlation was found between c-erb-B2, p53 and Ki-67 in OSCC (p>0.05). These findings suggested that the expression of p53 and increase of Ki-67 index may play roles in carcinogenesis.

KEYWORDS

Oral Squamous Cell Carcinoma; Dysplasia; Ki-67; p53; c-erb-B2; Immunohistochemical; Carcinogenesis.