Plectin, A Cytolinker Protein, Plays an Important Role in Differentiation and Actin Ring Formation in Osteoclasts

¹Division of Molecular Signaling and Biochemistry, Department of Health Improvement, Kyushu Dental University, 2-6-1, Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, Japan.
²Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, Sendai, Japan.
Corresponding Author: Takuma Matsubara, Division of Molecular Signaling and Biochemistry, Department of Health Improvement, Kyushu Dental University, 2-6-1, Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, Japan, Tel: +233500016591
E-Mail: [email protected]

Received Date: 26 Dec 2017  
Accepted Date: 22 Jan 2018  
Published Date: 26 Jan 2018

Copyright © 2018 Matsubara T

Citation: Matsubara T, Kinbara M, Maeda T, Yoshizawa M, et al. (2018). Plectin, A Cytolinker Protein, Plays an Important Role in Differentiation and Actin Ring Formation in Osteoclasts. Mathews J Cytol Histol. 1(1): 006.

ABSTRACT

Osteoclasts resorb the bone matrix and maintain bone and calcium homeostasis. During bone resorption, osteoclasts form an actin ring and seal the bone matrix for bone resorption. The tyrosine kinase Src is essential for actin ring formation and bone resorption. However, the molecular mechanisms underlying the regulation of actin ring formation by Src is still unclear. A cytolinker protein, plectin, was identified as one of the proteins downstream of Src. Plectin is localized, along with Src, around the actin ring of osteoclasts. Plectin binds to and is phosphorylated by Src. Differentiation and actin ring formation were inhibited by plectin downregulation. These results suggest an important role for plectin in osteoclast differentiation and actin ring formation via phosphorylation by Src.

KEYWORDS
Osteoclast; Bone Resorption; Actin Organization; Src; Plectin.

ABBREVIATIONS

Src:Rous sarcoma oncogene; M-CSF: Macrophage colony stimulating factor; RANKL: Receptor activator NF-κB ligand; RANK:Receptor activator NF-κB; MAPK: Mitogen-activated protein kinase; NFATc1: Nuclear factor of activated T cells, cytoplasmic, calcineur in dependent 1; Pyk2: Proline-rich tyrosine kinase 2; p130Cas: Crk-associated substrate; qPCR: Quantitative polymerase chain reaction; PKC: Protein kinase C; JNK: c-Jun N-terminal kinase; Erk: Extracellular regulated MAP kinase.