Anti-Bacterial Mechanism for Metallic Ag+, Cu2+, Zn2+ Ions-Induced Bactertiolysis on Disruptive OM Lpp and PGN Inhibitive Elongations Against S. aureus and E. coli

2-3-6, Saido, Midori-Ku, Saitama-Shi, Saitama-Ken, Japan

*Corresponding author: Dr. Sci. Tsuneo Ishida, 2-3-6, Saido, Midori-Ku, Saitama-Shi, Saitama-Ken, 〒336-0907, Japan, Phone: 048-881-3970; Email: [email protected]

Received Date: October 04, 2022

Published Date: November 04, 2022

Citation: Ishida T. (2022). Anti-Bacterial Mechanism for Metallic Ag⁺, Cu²⁺, Zn²⁺ Ions-Induced Bactertiolysis on Disruptive OM Lpp and PGN Inhibitive Elongations Against S. aureus and E. coli. Mathews J Cytol Histol. 6(1):18.

Copyrights: Ishida T. © (2022).

ABSTRACT

Anti-bacterial mechanism for complete-ionized Ag⁺, Cu²⁺, Zn²⁺ ion solutions has been established against S. aureus and E. coli. Anti-bacterial mechanism against S. aureus is involved that bacterolysis and destruction of S. aureus cell wall occur by inhibition of PGN elongation through metallic Ag⁺, Cu²⁺, Zn²⁺ ions-induced PGN inhibitive transglycosylase (TG) and transpeptidase (TP) syntheses (TG for Zn²⁺) and PGN activated major autolysin of amidase. The other, anti-bacterial mechanism against E. coli has been clarified that bacteriolysis and destruction of E. coli cell wall occur by disruption of E. coli outer membrane (OM) structure with OM lipoprotein-endopeptidase activation, and by inhibition of PGN elongation through inhibitive TG and TP syntheses (TG for Zn²⁺) and PGN activated major autolysins. Ag⁺, Cu²⁺, Zn²⁺ ions-induced ROS generation of O₂- and H₂O₂ and ROS-mediated oxidative stress in bacterial cell lead to killing by stress damage for silver ions, cell membrane damages due to high reactive •OH and OH^－ are formed by Haber-Weiss and Fenton reactions for Cu²⁺ ions, and DNA molecular damage for Zn²⁺ ions.

Keywords: Ag⁺, Cu²⁺, Zn²⁺ ions, Bacteriolysis, PGN synthesis and autolysin, PGN elongation, Autolysin amidase, ROS-mediated oxidative stress